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. 2021 Mar 5;12:628364. doi: 10.3389/fimmu.2021.628364

Figure 1.

Figure 1

hcmv-miR-US33as-5p inhibited the expression of IFNAR1 by targeting the 3′-UTR of IFNAR1. (A) Reporter vectors containing the 3′-UTRs of 13 predicted target genes were co-transfected with GV251 blank vector or vector containing US33as-5p into 293 cells. The dual-luciferase reporter assay was used to determine the relative Renilla luciferase activity normalized to firefly luciferase activity in cells in the corresponding wells at 48 h post-transfection (hpi) when cell lysates were harvested. (B) The expression of hcmv-US33as-5p during HCMV infection at different time points was determined by qPCR. U6 was used as a loading control. (C) IFNAR1 protein expression at different time points after the transfection of US33as-5p mimics was examined by western blot analysis. The sample of the control group comes from cells transfected with NC-RNA for 72 h. β-Actin was used as a loading control. The assays were performed in triplicate wells, and data were collected from two different experiments and are represented as the means ± SDs; **p < 0.05.