Figure 4.

hcmv-miR-US33as-5p inhibits the nuclear translocation of STAT1. (A) 293 cells were transfected with GV251 blank vector or vector containing US33as-5p, followed by selection with G418 (0.5 μg/mL) for 3 days. The cells were then transfected with the pDs-RED-STAT1 vector for 24 h and then stimulated with or without IFNα (1,500 IU/mL) for 12 h. STAT1 nuclear localization was detected by indirect immunofluorescence. (B) Percentage of cells with nuclear STAT1 from experiments performed in A. Cells were counted in three random fields and the results are expressed as mean ± SD (**p < 0.01). Fluorescence was visualized with an Olympus FluoView 1000 confocal microscope. All images were captured under a 60× objective lens.