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. 2021 Mar 5;12:628364. doi: 10.3389/fimmu.2021.628364

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Copyright © 2021 Zhang, Song, Ma, Lv, Zhang, Deng and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hcmv-US33as-5p is expressed in both viral latency and reactivation. (A) A schematic diagram depicting in vitro models of latent infection and viral reactivation. THP-1 cells were infected with WT or ΔmiRNA HCMV (MOI = 10) and maintained for 10 days in the culture medium. Then, the THP-1 cells were differentiated into macrophages (d-THP-1) by stimulated with TPA in order to render the cells permissive to HCMV infection and further maintained in the medium for another 5 days. (B) The expression of viral UL122 at 1, 3, 5, 7, and 9 dpi and 1, 3, and 5 days after TPA stimulation was detected by qPCR. (C) The expression of hcmv-US33as-5p at 9 dpi and 3 days after TPA stimulation was determined by qPCR.