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Cell type identification of IL-1β expressing cells. Double immunohistofluorescence staining for IL-1β (red) and F4/80 (green) confirmed that cells expressing IL-1β in response to systemic LPS injection were macrophages in the spleen (A) and choroid plexus (B), and Kupffer cells (or macrophages) in the liver (C). These cells expressed IL-1β 1 h after LPS injection. Note the high rate of IL-1β-immunopositive cells among all macrophages in all three different tissue types. Bars =20 μm.
