Table 1.
List of antibodies.
| Antibody against | Description | Host | Dilution | Source |
|---|---|---|---|---|
| α-SMA | smooth muscle cell | rabbit | 1:100 | AnaSpec (Fremont, CA,USA) |
| CCL2 | cytokine | rabbit | 1:100 | abcam (Cambridge, UK) |
| CD31 | vascular endothelium | rat | 1:200 | BMA Biomedicals (Augst, Switzerland) |
| CXCL1 | cytokine | goat | 1:100 | R&D Systems (Minneapolis, MN, USA) |
| CXCL2 | cytokine | rabbit | 1:100 | AbD Serotec, Bio-Rad (Hercules, CA, USA) |
| CXCR2 | cytokine receptor | rat | 1:50 | R&D Systems |
| ER-TR7 | fibroblast | rat | 1:200 | abcam |
| F4/80 | macrophage | rat | 1:100 | abcam |
| Iba-1 | microglia | rabbit | 1:1000 | Wako (Osaka, Japan) |
| IL-1R1 | cytokine receptor | goat | 1:20 | R&D Systems |
| IL-1RAcP | receptor accessory protein | goat | 1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
| IL-1β | cytokine | goat | 1:200 | R&D Systems |
| IL-6 | cytokine | rabbit | 1:100 | Bioss (Woburn, MA, USA) |
| IL-6R | cytokine receptor | goat | 1:20 | R&D Systems |
| NG2 | pericyte | rabbit | 1:100 | Merck Millipore (Burlington, MA, USA) |
| S-100 | arachnoid cell | rabbit | 1:100 | gift from Dr. K Katoh |
| transthyretin | choroid plexus epithelium | rabbit | 1:200 | Proteintech (Rosemont, IL, USA) |