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. 2021 Mar 10;8:520–528. doi: 10.1016/j.toxrep.2021.03.002

Table 2.

Combinations of primary antibodies for double immunofluorescence staining.

Antibody for cytokine (host) Antibodies for cell markers (host)
IL-1β (goat) F4/80 (rat)
IL-1β (goat) Iba-1 (rabbit)
IL-1β (goat) transthyretin (rabbit)
IL-1β (goat) CD31 (rat)
IL-1β (goat) NG2 (rabbit)
Antibody for receptor (host) Antibodies for cell markers (host)
IL-1R1 (goat) transthyretin (rabbit)
IL-1R1 (goat) ER-TR7 (rat)
IL-1R1 (goat) S100 (rabbit)
IL-1R1 (goat) NG2 (rabbit)
IL-1R1 (goat) F4/80 (rat)
IL-1R1 (goat) α-SMA (rabbit)
IL-1R1 (goat) CD31 (rat)
IL-1R1 (goat) Iba-1 (rabbit)
Antibodies for receptors (host) Antibodies for cytokines (host)
IL-1R1 (goat) IL-6 (rabbit)
IL-1R1 (goat) CCL2 (rabbit)
IL-1R1 (goat) CXCL2 (rabbit)
IL-1RAcP (goat) CXCL2 (rabbit)
CXCR2 (rat) CXCL1 (goat)
Antibody for receptor (host) Antibody for receptor (host)
IL-1R1 (goat) CXCR2 (rat)