Figure 11.

Coexpression of Cx30 has no apparent effect on the single-channel current amplitude and open probability of ENaC.A and B, representative continuous single-channel current recordings in an outside-out patch of an ENaC expressing oocyte (A) or an ENaC+Cx30 coexpressing oocyte (B) obtained at a holding potential of −70 mV. Application of amiloride (2 μM) is indicated by filled bars. The current level at which all channels are closed (C) was determined in the presence of amiloride. The channel open levels are indicated by (1) and (2). Single-channel binned current amplitude histograms were obtained by analyzing an 85 s portion of the current trace indicated by a horizontal line and were used to calculate single-channel current amplitude (i) and P_{O (app)} values. C and D, summary of data from similar experiments as shown in A and B. Mean ± SEM and data points for individual oocytes are shown; n.s., not significant; Mann–Whitney test (n = 7, N = 4).