Figure 12.

Intact C-termini of β- and γENaC are necessary for the inhibitory effect of Cx30 on ENaC.A, normalized ΔI_Ami values are shown from oocytes expressing wild-type ENaC (αβγ), truncated αENaC (P619X), βENaC (R566X), or γENaC (K576X) with corresponding wild-type ENaC subunits (α_Tβγ, αβ_Tγ or αβγ_T) or triple-truncated ENaC (α_Tβ_Tγ_T) in each case with or without Cx30. Individual ΔI_Ami values were normalized to the mean ΔI_Ami obtained in matched oocytes from the same batch expressing wild-type ENaC without Cx30. Mean ± SEM and data points for individual oocytes are shown; ∗∗∗p < 0.001; ∗∗p < 0.01; n.s. not significant; Kruskall–Wallis with Dunn’s post hoc test (67 ≤ n ≤ 153, 5 ≤ N ≤ 7). B, relative inhibitory effect of Cx30 on wild-type (αβγ) or truncated (α_Tβγ, αβ_Tγ, αβγ_T or α_Tβ_Tγ_T) ENaC calculated as described in Figure 3 using original data shown in A. ΔI_Ami obtained in oocytes expressing wild-type (αβγ) or truncated (α_Tβγ, αβ_Tγ, αβγ_T, or α_Tβ_Tγ_T) ENaC together with Cx30 was normalized to the corresponding mean ΔI_Ami recorded in matched control oocytes from the same batch expressing wild-type (αβγ) or truncated (α_Tβγ, αβ_Tγ, αβγ_T or α_Tβ_Tγ_T) ENaC alone. Mean ± SEM and data points for individual oocytes are shown; ∗∗∗p < 0.001; n.s., not significant; Kruskall–Wallis with Dunn’s post hoc test.