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. 2021 Feb 10;296:100404. doi: 10.1016/j.jbc.2021.100404

Figure 14.

Figure 14

ENaC inhibition by Cx30 does not involve a Nedd4-2-dependent mechanism.A, ΔIAmi values obtained in oocytes expressing wild-type ENaC (αβγ), mutant ENaC (αβγP623A-P625A, αβγY627A, αY644AβY620AγY627A), or coexpressing wild-type ENaC together with xNedd4-CS (αβγ + xNedd4-CS) in each case with (+) or without (−) additional coexpression of Cx30 were normalized as described in Figure 12A. Mean ± SEM and data points for individual oocytes are shown; ∗∗∗p < 0.001; ∗∗p < 0.01; Kruskall–Wallis with Dunn’s post hoc test (32 ≤ n ≤ 185, 3 ≤ N ≤ 4). B, relative inhibitory effect of Cx30 on wild-type ENaC (αβγ), mutant ENaC (αβγP623A-P625A, αβγY627A, αY644AβY620AγY627A), or wild-type ENaC coexpressed with xNedd4-CS (αβγ + xNedd4-CS) calculated as described in Figure 12B using original data shown in A. Mean ± SEM and data points for individual oocytes are shown; ∗p < 0.05; ∗∗∗p < 0.001; n.s., not significant; Kruskall–Wallis with Dunn’s post hoc test.