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. 2021 Mar 5;9:629895. doi: 10.3389/fcell.2021.629895

FIGURE 3.

FIGURE 3

METTL3 promotes osteogenic differentiation of primary ligament fibroblasts by m6A methylation of lncRNA XIST. (A) The expression of LINC00657, POLDIP2, OIP5-AS1, MALAT1, NEAT1, XIST, and SLC26A4-AS1 in primary ligament fibroblasts from patients with OPLL transduced with indicated plasmids. (B,C) The expression of LINC00657 and XIST in ligament tissues from controls (n = 20) and patients with OPLL (n = 20). (D,E) Pearson correlation scatter plots in ligament tissues from patients with OPLL (n = 20). (F,G) The levels of XIST m6A and expression, (H) Alizarin Red S staining, and (I) the expression of ALP and COL1 in primary ligament fibroblasts from patients with OPLL transduced with indicated plasmids. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.