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. 2021 Mar 5;9:629895. doi: 10.3389/fcell.2021.629895

FIGURE 4.

FIGURE 4

XIST functions as a miR-302a-3p sponge in primary ligament fibroblasts. (A) The subcellular fractions of XIST. (B) Potential binding sites for miR-302a-3p, miR-302b-3p, miR-302c-3p, miR-302d-3p, and miR-302e in XIST. (C) miRNA expression in primary ligament fibroblasts from patients with OPLL transduced with indicated plasmids. (D) Expression of XIST or miR-302a-3p associated with AGO2. (E) Luciferase assays of the binding of XIST and miR-302a-3p. (F) The expression of miR-302a-3p. (G) Pearson correlation analysis. (H) Alizarin Red S staining and (I) the expression of ALP and COL1 in primary ligament fibroblasts from patients with OPLL transduced with indicated plasmids. Scale bar: 100 μm. *P < 0.05, ***P < 0.001.