Figure 3.

Comparison of three different coCFTR sequences

Different approaches to codon optimization were used to generate coCFTR1, coCFTR2, and coCFTR3. (A) CFTR is composed of five distinct protein domains, including two transmembrane domains (TMD1 and TMD2), two nucleotide-binding domains (NBD1 and NBD2), and a regulatory domain (RD). (B) The percent similarity of codons compared to WT CFTR is shown by protein domain. (C) The individual nucleotide content for each sequence was quantified. (D) The percentage of codons using a G or C at the third nucleotide position (GC₃) for each sequence is shown by protein domain.