Table 1.

Codon usage of CFTR sequences

				WT CFTR	coCFTR1a	coCFTR2b	coCFTR3
Ala	G	C	G	6	3	4	0
			A	34	26	20	0
			T	27	20	22	0
			C	16	34	37	83
Arg	G	C	G	16	13	17	0
			A	37	24	22	0
	C	G	G	8	4	13	0
			A	9	33	7	0
			T	3	2	7	0
			C	5	2	12	78
Asn	A	A	T	25	21	24	0
			C	29	33	30	54
Asp	G	A	T	38	32	26	0
			C	20	26	32	58
Cys	T	G	T	8	6	10	0
			C	10	12	8	18
Gln	C	A	G	31	37	47	67
			A	36	30	20	0
Glu	G	A	G	30	45	61	93
			A	63	48	32	0
Gly	G	G	G	16	10	21	0
			A	38	30	18	0
			T	15	14	13	0
			C	15	30	32	84
His	C	A	T	11	9	7	0
			C	14	16	18	25
Ile	A	T	A	33	26	14	0
			T	50	39	46	0
			C	36	54	59	119
Leub	T	T	G	34	27	29	0
			A	37	29	13	0
	C	T	G	37	26	72	183
			A	19	12	9	0
			T	32	20	23	0
			C	24	69	38	0
Lys	A	A	G	35	47	53	92
			A	57	45	39	0
Meta	A	T	G	38	39	38	38
Pheb	T	T	T	47	35	37	0
			C	38	50	47	85
Pro	C	C	G	1	0	5	0
			A	13	21	12	0
			T	20	15	13	0
			C	11	9	15	45
Ser	A	G	T	19	15	18	0
			C	24	20	28	123
	T	C	G	4	3	9	0
			A	29	22	24	0
			T	30	23	26	0
			C	17	40	18	0
Thr	A	C	G	3	3	6	0
			A	33	31	18	0
			T	32	29	26	0
			C	15	20	33	83
Trp	T	G	G	23	23	23	23
Tyr	T	A	T	22	15	15	0
			C	18	25	25	40
Vala	G	T	G	36	46	48	89
			A	12	11	9	0
			T	23	17	12	0
			C	18	14	20	0
Stop	T	G	A	0	0	0	0
		A	G	1	1	1	0
			A	0	0	0	1
			Total	1481	1481	1481	1481
			CAI	0.74	0.76	0.80	0.99

The codon adaptive index (CAI) is higher in all coCFTR sequences compared to WT CFTR. coCFTR1 and coCFTR2 were codon optimized with single amino acid changes that do not affect protein function.

^acoCFTR1 substitutes methionine for valine at position 1475.

^bcoCFTR2 substitutes leucine for phenylalanine at position 833.