Figure S1.

Determination of the effects of α-cell GCK activation on glucose homeostasis in adult female mice. (A) Body weights of α-cell GCK mutant and control mice (n = 5 for α-mutGCK, n = 10 total for Gck^{LoxPGck∗/LoxPGck∗} and Gcg-CreER^T2). (B)Ad libitum blood glucose for α-cell GCK mutant and control mice (n = 5 for α-mutGCK, n = 10 total for Gck^{LoxPGck∗/LoxPGck∗} and Gcg-CreER^T2). (C) Intraperitoneal glucose tolerance test (1 g/kg bodyweight) (n = 5 for α-mutGCK, n = 10 total for Gck^{LoxPGck∗/LoxPGck∗} and Gcg-CreER^T2). (D) Area under the curve (AUC) and (E) fasted blood glucose measurements for intraperitoneal glucose tolerance test in (C). (F) Plasma glucagon and (G) plasma insulin in fasted or 5 min after glucose injection in (C). (∗p < 0.05 vs. genetically unmodified control). Analysis by two-way ANOVA with post hoc Bonferroni test.