Figure 3.

Activation of α-cell GCK impairs the counter-regulatory response during an insulin tolerance test.(A) Intraperitoneal insulin tolerance test (0.75 U/kg body weight) in adult male mice (n = 5 for α-mutGCK, n = 7 total for Gck^{LoxPGck∗/LoxPGck∗} and Gcg-CreER^T2). (B) Plasma glucagon in mice fasted or 30 min after insulin injection in (A). (C) Intraperitoneal insulin tolerance test in adult female mice (0.75 U/kg bodyweight) (n = 3 for α-mutGCK, n = 7 total for Gck^{LoxPGck∗/LoxPGck∗} and Gcg-CreER^T2). (D) Plasma glucagon in mice fasted or 30 min after insulin injection in (C). (∗p < 0.05, ∗∗p < 0.01 vs. genetically unmodified control). Analysis by two-way ANOVA with post hoc Bonferroni test.