Fig. 3.

Rescue and identification of XJ11129 in vitro. A Visible cytopathic effect (CPE) of BHK-21 cells in 2–3 days after infection with recued virus. B Plaque morphology of rescued XJ11129 and the parental XJ11129. Plaques were observed 3 days post-infection after staining with 0.2% crystal violet. C The GA products were inoculated to BHK-21 cells for incubation of 96 h. The infected cells were observed with a fluorescence microscope. D Differences between the sequences of rXJ11129 with its parental virus XJ11129. The numeral subscript after nucleotide is the location of whole viral genome. E Growth kinetics of rXJ11129. The experiments were performed with BHK-21 cells, Vero cells, C6/36 cells and DF-1 cells. Viral titres were determined on BHK-21 cells by plaque assay at the indicated times. The dotted lines represent the limit of sensitivity of the plaque assay. The error bars indicate the range in values of two independent experiments.