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TDP-43 is cleaved by the 3C protease of EV-A71. A HeLa cells were cultured in 12-well plates to 60% confluency and infected with EV-A71 or transfected with pEGFP-2A or pEGFP-3C for 24 h. Control cells were treated with PBS. Cell lysates were subjected to Western blot analysis. B Cells were transfected with pEGFP-3C for various times. Control cells were transfected with pEGFP-C1. Cell lysates were analyzed by Western blotting.
