Table 1.
Flowering-time candidate genes and coinciding SV identified between 7 elite parental lines within QTL for flowering time analyzed in a B. napus multiparental elite population (n = 354)
QTL name | Size of LD block in kb | Start–end position | Number of genes/flowering-time genes in Darmor-bzh | Number of genes/flowering-time genes with structural variants* | Gene IDs | Blast2GO annotation of gene | Name: size and type of SV (relative to Darmor-bzh) | PCR assay name: product size polymorphism (see Fig. 3) |
---|---|---|---|---|---|---|---|---|
A02_1 | 352 | 12,297–364,239 | 92/10 (10.9%) | 14 / 2 (14.2%) | BnaA02g00160D; BnaA02g00910D | Pollen development; regulation of flower development | SV1: 373 bp deletion; SV2: 64 bp deletion | P133: 1130 bp/757 bp; P255: 419 bp/362 bp |
A02_2 | 498 | 6,311,854–6,810,319 | 59/8 (13.6%) | 6/1 (16.7%) | Promoter of BnaFT.A02; BnaA02g12130D (BnaFT.A02) | -; photoperiodism, flowering, positive regulation of flower development | SV3: 1,361 bp deletion; SV4: 288 bp deletion | P311: 1924 bp/563 bp; P416: 797 bp/519 bp |
A02_3 | 451 | 22,569,685–23,021,163 | 73/3 (4.1%) | 2/0 (0%) | – | – | – | – |
A02_4 | 383 | 24,112,932–24,495,807 | 72/11 (15.3%) | 5 / 2 (40%) | BnaA02g33650D; BnaA02g33940D | Circadian rhythm; entrainment of circadian clock | SV5: 1,313 bp deletion; SV6: 35 bp deletion | P513: 819 bp/no product; P632: 667 bp/632 bp |
C02_1 | 331 | 512,169–842,796 | 70/0 (0%) | 2/0 (0%) | – | – | – | – |
C02_2 | 860 | 1,028,042–1,888,045 | 153/11 (7.2%) | 2/1 (50%) | BnaC02g03640D | Regulation of flower development | SV7: 38 bp insertion | P732: 262 bp/300 bp |
C02_3 | 360 | 3,168,965–3,528,564 | 62/5 (8.1%) | 10/0 (0%) | – | – | – | – |
C03_1 | 914 | 2,434–915,986 | 190/23(12.1%) | 11/0 (0%) | – | – | – | – |
Sum | 4,149 | - | 771/71 (9.2%) | 52/6 (11.5%) | – | – | – | – |
Flowering-time genes were defined according to Schiessl et al. (2015) as genes associated by Blast2GO with at least one of the following gene ontology terms: flower, vernalization, photoperiod, circadian, floral, vegetative to reproductive, vegetative phase change, pollen carpel, sepal, petal