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. 2021 Mar 18;11:6268. doi: 10.1038/s41598-021-85241-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adhesion of AGS and NUGC3 gastric cancer cells to vascular endothelial cells. (a) Representative images of the adhesion of tumour cells (AGS, upper; NUGC3, lower) to monolayer-cultured HUVEC stimulated by 1.0 µg/mL of LPS and 100 µg/mL of MP. Scale bars are equal to 500 µm. (b) Number of tumour-binding cells (AGS and NUGC3) to monolayer-cultured HUVEC stimulated by 1.0 µg/mL of LPS and 100 µg/mL of MP. Data are means ± standard error (n = 6, each group); *P < 0.05. (c) Representative images of the adhesion of tumour cells (AGS, upper; NUGC3, lower) to monolayer-cultured HHSEC stimulated by 1.0 µg/mL of LPS and 100 µg/mL of MP. Scale bars are equal to 500 µm. (d) Number of tumour-binding cells (AGS and NUGC3) to monolayer-cultured HHSEC stimulated by 1.0 µg/mL of LPS and 100 µg/mL of MP. Data are means ± standard error (n = 6, each group); *P < 0.05. (e) Representative images of the adhesion of tumour cells (AGS, upper; NUGC3, lower) to monolayer-cultured HUVEC depleted of E-selectin stimulated by 1.0 µg/mL of LPS. Scale bars are equal to 500 µm. (f) Number of tumour-binding cells (AGS and NUGC3) to monolayer-cultured HUVEC depleted of E-selectin stimulated by 1.0 µg/mL of LPS. Data are means ± standard error (n = 6, each group); *P < 0.05. (g) Invasion assay of of AGS and NUGC3 gastric cancer cells co-cultured with HUVEC stimulated by 1.0 µg/mL of LPS and 100 µg/mL of MP. Data are means ± standard error (n = 5, each group); *P < 0.05.