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. 2021 Mar 18;11:6268. doi: 10.1038/s41598-021-85241-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of E-selectin expressed in the serum and portal vein following MP administration in an LPS-induced murine hepatic metastasis model. (a) ELISA analysis of serum E-selectin levels at 6 h after stimulation. Data are means ± standard error (n = 4, each group); *P < 0.05. n.s. no significance. (b–d) Analysis of serum cytokine levels at 1, 3, and 6 h after stimulation with LPS and MP. ELISA analysis of serum concentrations of IL-6 (b), TNF-α (c), and IL-1β (d). Data are means ± standard error (n = 4, each group); *P < 0.01. (e) Western blotting analysis of E-selectin protein expression levels in the portal vein at 6 h after stimulation. Blot was cut horizontally and immunoblotting was performed for each section. (f) Quantification of E-selectin band intensity normalized to β-actin. Data are means ± standard error (n = 3, each group); *P < 0.05. (g) Induction of Sele gene expression at 6 h after stimulation was analyzed by quantitative real-time PCR. Data are normalized relative to Actb mRNA levels. Data are means ± standard error (n = 4, each group); *P < 0.05.