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. 2021 Mar 18;11:6277. doi: 10.1038/s41598-021-85644-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examination of IAA uptake based on heterologously transformed yeast. (A) Rc5NG4-1Y- or Rc5NG4-1C-fused GFP were expressed in yeast cell and fluorescence observed by confocal microscopy. Scale bar = 2 μm. (B) Rc5NG4-1Y and Rc5NG4-1C expression in Rc5NG4-1Y transformed yeast, Rc5NG4-1C transformed yeast and empty vector pDR196 transformed yeast detected by RT-PCR. The upper group and lower group of blots were cropped from different part of same gels, and full-length blots are presented in Figure S4. (C) ²H-IAA uptake of the different transformed yeast strains. The values are means ± SD of three replications (transformations carried out once for each and then grow in triplicate).