Figure 2.

Stimulation of male-like development in female germ cells growing in XX pseudotestes by Phf7. (a,b), Δtra/tra¹, nos-Gal4 pseudotestes. (a), sparse type, (b), abnormal type. (c,d), UAS-Phf7.N, Δtra/tra¹, nos-Gal4 pseudotestes. (c), sparse type, (d), abnormal type. E–G, UAS-Phf7.FL, Δtra/tra¹, nos-Gal4 pseudotestes. (e), sparse type, (f), abnormal type, (g), partial rescue type. (h), tra¹, nos-Gal4/ + testis as a normal control. (a–h), Vasa in green, N-cadherin in red. (i–l), comparison between “partial rescue” pseudotestes and normal testes. (i,j) show Vasa staining, (k–l) show the DAPI signals. (m), distribution of different types of pseudotestes under various conditions. Blue, orange, and purple bars indicate the sparse, abnormal, and partial rescue categories respectively. Sample sizes are indicate for each genotype on the graph. The temperatures at which each set of data was performed under as well as the Phf7 construct expressed are indicated below the graph. N is UAS-Phf7.N and FL is UAS-Phf7.FL.