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. 2021 Mar 18;11:6308. doi: 10.1038/s41598-021-85560-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of sex-specific markers in the pseudotestes. (a–d), Sxl staining in wild-type ovaries (a), wild-type testis (b), and abnormal (c) and partial rescue (d) type pseudotestes of UAS-Phf7.FL, Δtra/tra¹, nos-Gal4. Vasa is in red, Sxl in green, and N-cadherin in blue. (a′–d′) display just the Sxl signals alone. The yellow arrow in A′ indicate the germline stem cells in an ovariole that stain Sxl clearly. (e–h), Staining of Bam in wild-type ovary (e), wild-type testis (f), and abnormal (g) and partial rescue (h) type pseudotestes of UAS-Phf7.FL, Δtra/tra¹, nos-Gal4. Vasa is in red, Bam in green, and N-cadherin in blue. (i–k), Phf7 expression in wild-type testes (i), and abnormal (j) and partial rescue (k) type pseudotestes of UAS-Phf7.FL, Δtra/tra¹, nos-Gal4. Vasa is in red, Phf7 in green, and Armadillo in blue. (i′–k′) display the Phf7 channel alone.