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. 2021 Mar 18;11:6305. doi: 10.1038/s41598-021-85802-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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T2-weighted fluid attenuation inversion recovery (FLAIR) images of three participants with World Health Organization (WHO) grade II, III, and IV glioma (left). Red boxes indicate regions of FLAIR signal abnormality biopsied for pathologic examination and cell-counting with Raman scattering microscopy (right). Pseudo-H&E images of fresh biopsy specimens demonstrate ablation of normal cytoarchitecture across all WHO tumor grades. Here, lesion cellularity and necrotic features escalate with increasing WHO grade.