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. 2021 Mar 18;11:6355. doi: 10.1038/s41598-021-85798-y

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rapid time-course analysis of relationships between changes in oxygen levels in the nucleus accumbens (NAc) and subcutaneous (SC) space induced by arousing stimuli in awake, freely moving rats. (A) Mean (± SEM) changes in oxygen levels relative to baseline (100%) calculated with 4-s time bins. Vertical hatched lines show onset and offset of stimulus presentation. Filled symbols mark values significantly different from pre-stimulus baseline (P < 0.05). (B) Correlative relationships between oxygen changes in two recording locations shown for the time interval between the stimulus onset and maximal oxygen increase (duration of this interval is shown in brackets). Data were obtained in 6 rats; the numbers of averaged responses and F values of one-way repeated measure ANOVAs are shown in Supplementary materials.