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. 2021 Mar 18;11:6281. doi: 10.1038/s41598-021-85740-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protocols for animal experimentation and morphometrical analysis. (A) Outline of low-density lipoprotein receptor knockout (LDLR −/−) minipigs experiment. (B–E) Images of coronary arteries from LDLR −/− minipigs before stent implantation. (B) Angiographically, mild stenosis due to arteriosclerosis is visible. (C–E) Eccentric fibro-fatty plaques (arrows) are observed on intravascular ultrasound. (F–I) Grading of peri-strut inflammation for semi-quantitative analysis. (F) Grade 0, absent, few inflammatory cells. (G) Grade 1, mild, fewer than 20 inflammatory cells per high power field (HPF). (H) Grade 2, moderate, fewer than 50 inflammatory cells per HPF. (I) Grade 3, severe, more than 50 inflammatory cells per HPF. (J) Tissue characteristics are evaluated for the neointima above the stent strut and the neointima distant from the stent struts. (K, L) All sections of stented regions harvested from porcine coronary arteries are immunostained for anti-CD31 antibody. The total luminal circumference (K) was measured and the lengths of re-endothelialized regions (arrows in L) were added up. The ratio of the re-endothelialized region was calculated (F–J; hematoxylin–eosin, K, L; immunohistochemistry for anti-CD31 antibody. C, D *; stent strut).