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. 2021 Mar 8;22(2):215–236. doi: 10.1007/s10522-021-09913-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Nematode’s age at time of test performance and exposure initiation. The age of the nematodes at the day of test performance (or test initiation, respectively, for survival-based tests), were summarized for assays analyzing stress resistance (including heat, oxidative, pathogenic, UV, and osmotic stress resistance), locomotion (on solid and in liquid media), pharyngeal pumping, ROS level, translocation of DAF-16, and HSPs/SODs/GSTs (including the activity, quantity or expression of these genes c.q. proteins). The percentage distribution was calculated according to the total number of age groups investigated per phenotype (a). In addition, the most popular exposure periods to treat the nematodes with the test compounds were determined. The graph represents the distribution of exposure scenarios used in the collected studies in the database (b). A1-A10 = 1st–10th day of adulthood; L1-L4 = 1st–4th larval stage