Table 2.
Immunohistochemistry of the antibodies and methodology. MAP1S microtubule-associated protein 1S, pAMKα phosphorylated adenosin-5′ monophosphate activated kinase a, LC3 microtubule-associated protein 1A/1B-light chain 3, SIRT1 silent information regulator T1, TTF1 thyroid transcription factor 1, CK 5/6 cytokeratin 5/6, GLUT1 glucose transporter type 1, LDH lactate-dehydrogenase
| Antibody | P16 INK4A | P21 | β-Galactosidase | MAP1s | PAMPKα | LC3 | SIRT1 | TTF1 | CK 5/6 | GLUT1 | LDH |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Producer | ENZO | DAKO | Abcam | Novus | Cell Signaling | Nano-tools | LSBio | DAKO | DAKO | Neo-markers | Abcam |
| Clone | ENZ-ABS-377-0100 | SX118 | Ab4761 | NBP2-47383 | THr172 | 0231-100/LC3-5F10 | LS-B1895 | M3575 | B4rtu (DAKO GA780) | RB-9052-P | EP1566Y ab52488 |
| Dilution | 1:1000 | 1:30 | 1:1500 | 1:1000 | 1:100 | 1:200 | 1:500 | 1:100 | Ready to use | 1:200 | 1:100 |
| Pretreatment | CC1 Ventana Ultra | MW9,0 (DAKO S2367) | CC1 Ventana Ultra | CC1 Ventana Ultra | CC1 Ventana Ultra | MW9,0 (DAKO S2367) | MW9,0 (DAKO S2367) | MW9,0 (DAKO S2367) | Flex high pH | MW Natrium-citrat 6,0 | MW9,0 (DAKO S2367) |
| Detection kit | Ventana optiviev+ amplification | Envision DAKO K5007 | Ventana optiviev + amplification | Ventana Ultra-View | Ventana Ultra-View | Envision DAKO K5007 | Envision DAKO K5007 | Envision DAKO K5007 | HRP (GV800) | Envision DAKO K5007 | Envision DAKO K5007 |