Table II.
Gene | Primer sequence (5–3) |
---|---|
miR-181a | F: CGGTAACATTCAACGCTGTCG |
R: GTGCAGGGTCCGAGGT | |
TGF-β1 | F: ATGGTGGAAACCCACAACGAA |
R TGCTGAGGTATCGCCAGGAAT | |
Smad4 | F: CCATTTCCAATCATCCTGCTC |
R: GAAGGGTCCACGTATCCATCA | |
E-cadherin | F: TTGTGGCAGAGTGTAATGCTG |
R: GTCCCTGGTCTTCTTGGTCA | |
Vimentin | F: AGAGAACTTTGCCGTTGAAGC |
R: ACGAAGGTGACGAGCCATT | |
U6 | F: CGCTTCGGCAGCACATATA |
R: TTCACGAATTTGCGTGTCAT | |
GAPDH | F: CATGAGAAGTATGACAACAGCCT |
R: AGTCCTTCCACGATACCAAAGT |
miR, microRNA; F, forward; R, reverse.