Figure 3.

Effects of hsa_circ_0072309 overexpression on PPARγ/PTEN and PI3K/AKT signaling. (A) The expression levels of proteins including PPARγ, PTEN, p/t-PI3K, p/t-AKT and p/t-mTOR were determined using western blotting. (B) The mRNA expression levels of PPARγ, PTEN, PI3K, AKT and mTOR were detected by reverse transcription-quantitative PCR. Error bars represent the mean ± SEM from three independent experiments. ***P<0.001 vs. control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. Oe-circ_0072309; ^$$$P<0.001 vs. pioglitazone + GW9662; ^ΔP<0.05, ^ΔΔP<0.01, ^ΔΔΔP<0.001 vs. Oe-circ_0072309 + pioglitazone. circ, circular RNA; Oe, overexpression vector; PPARγ, peroxisome proliferator-activated receptor γ; p-, phosphorylated; t-, total.