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. 2021 Mar 8;23(5):328. doi: 10.3892/mmr.2021.11967

Figure 1.

Figure 1.

miR-301a-3p promotes NPC proliferation in vitro. (A) The expression of miR-301a-3p in two NPC cell lines (C666-1 and 5–8F) and the NP69 cell line was examined via RT-qPCR. (B) RT-qPCR analysis of miR-301a-3p in C666-1 and 5–8F cells after transfection of miR-301a-3p mimic or NC. (C) The proliferation of C666-1 and 5–8F cells after transfection with the miR-301a-3p mimic or NC was assessed using the Cell Counting Kit-8 assay. (D) EdU assay of C666-1 and 5–8F cells transfected with miR-301a-3p mimic or NC. Magnification, ×200. (E) Representative images (left) and quantification (right) of the indicated cells subjected to the colony-formation assay. (F) miR-301a-3p inhibition prevents C666-1 cell proliferation and decreases EdU-positive rate and clonogenic potential. Magnification, ×200. *P<0.05; **P<0.01 vs. Control group. NPC, nasopharyngeal carcinoma cell; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; in, inhibitor.