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Multicolor BUV/BV fluorochrome analysis withUV lasers. (a) Analysis of mouse splenocytes (frozen/thawed) labeled with BUV496 anti-mouse B220, BUV563 anti-mouse CD4, BUV661 anti-mouse CD19, BV421 anti-mouse CD3 and BV510 anti-mouse CD8a with 355 nm (top scatterplots) or 320 nm (bottom scatterplots) laser sources. Compensation matrices (FlowJo) are shown for each five color analysis.
