FIGURE 2.

Constrained phospholipids can be used to form stable LNPs. 120 LNPs were formulated by combining constrained phospholipids with (a) three sterol variants, C14PEG2000, and cKK‐E15 at (b) four different formulation ratios. (c) 109 LNPs passed quality control criteria and had a diameter less than 200 nm as well as a stable autocorrelation curve. LNP diameter was not impacted by the presence of (d) different hydrocarbon tails or (e) sterol variant used. (f) The average diameter of the 109 LNP pool administered to mice was within range of the individual LNPs that make up the pool