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. 2021 Mar 3;23(5):317. doi: 10.3892/mmr.2021.11956

Figure 1.

Figure 1.

Characterization of mouse Tex33. (A) Analysis of the Tex33 gene in mice. The Tex33 gene consists of seven exons. (B) Tex33 transcript variants in mice. Deleted regions reveal alternate splicing events and different combinations of exons. Transcript variant V1 contains six exons, transcript variant V2 has seven exons and transcript variant V3 has five exons. (C) Two different isoforms are encoded by transcript variants V1, V2 and V3. (D) mRNA levels of Tex33 transcript variants in various mouse tissues were analyzed by RT-PCR. Transcript variant V1, 220 bp; transcript variant V2, 348 bp; transcript variant V3, 261 bp. (E) Expression of TEX33 protein in various mouse tissues, as determined by western blot analysis. (F) Expression of Tex33 transcript variants during the first wave of spermatogenesis, as determined by RT-PCR. (G) Expression of TEX33 protein during the first wave of spermatogenesis, as determined by western blot analysis. TEX33, testis-expressed protein 33; RT-PCR, reverse transcription-polymerase chain reaction.