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. 2021 Mar 3;23(5):317. doi: 10.3892/mmr.2021.11956

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Copyright: © Xia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Localization of TEX33 during spermatogenesis. (A) Immunofluorescence staining of TEX33 in the seminiferous tubules during different stages of spermatogenic development (stages I–XII). (B) Co-immunofluorescence staining of TEX33 with PNA-FITC in the seminiferous tubules during different stages of spermatogenic development. (C) Co-immunofluorescence staining of TEX33 and α-tubulin in elongating spermatids during spermatogenic stage X. (D) Co-immunofluorescence staining of TEX33 with PNA-FITC in sperm. TEX33, testis-expressed protein 33; PNA-FITC, FITC-conjugated peanut agglutinin; DAPI, 4,6-diamidino-2-phenylindole; M, manchette (indicated by arrowheads).