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. 2021 Mar 3;23(5):317. doi: 10.3892/mmr.2021.11956

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Copyright: © Xia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Generation of Tex33 knockout mice. (A) Schematic representation of the deletion of Tex33 exons 2–4 via Cas9 microinjection. Primers were designed for genotyping. F targets intron 1, R1 targets exon 2 and R2 targets intron 4. (B) Genotyping of Tex33 offspring by PCR assay. (C) Comparison of TEX33 knockout efficiency between Tex33^+/+, Tex33^+/− and Tex33^−/− mice by western blot analysis. (D) Detection of TEX33 expression in testes (upper panels) and sperm (lower panels) by immunofluorescence analysis. WT, wild-type; F, forward; R, reverse; gRNA, guide RNA; TEX33, testis-expressed protein 33; +/+, Tex33^+/+ (wild-type) mice; +/−, Tex33^+/− (heterozygous) mice; −/−, Tex33^−/− (homozygote) mice; Bl, blank; DAPI, 4,6-diamidino-2-phenylindole.