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. 2021 Mar 3;23(5):317. doi: 10.3892/mmr.2021.11956

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Copyright: © Xia et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Phenotypic analysis of Tex33 knockout mice. (A) Testis and epididymis from Tex33^+/+ and Tex33^−/− males. Tissue samples were collected from 10-week-old male mice (n=5 per genotype). (B) Testis/body weight ratio of Tex33^+/+ and Tex33^−/− males. Values are expressed as mean ± standard error of mean. (C) H&E staining showing seminiferous tubules with normal histology in Tex33^+/+ and Tex33^−/− males. (D) Periodic acid-Schiff staining showing normal acrosome development and spermatogenesis in Tex33^+/+ and Tex33^−/− males. (E) Co-immunofluorescence staining of TEX33 and α-tubulin in elongating spermatids revealed normal manchette morphology in both Tex33^+/+ and Tex33^−/− males. H&E staining of Tex33^+/+ and Tex33^−/− males showing normal (F) cauda epididymis and (G) sperm. (H) H&E staining revealed normal ovarian morphology in Tex33^+/+ and Tex33^−/− females. H&E, hematoxylin and eosin; Tex33, testis-expressed protein 33; +/+, Tex33^+/+ (wild-type) mice; −/−, Tex33^−/− (homozygote) mice; M, manchette (indicated by arrowheads); DAPI, 4,6-diamidino-2-phenylindole; N.S., not significant.