Figure 2.

LAMP2A is highly expressed in human CRC cell lines. (A) LAMP2A expression in normal colonic epithelial cells, HCT116/DLD-1 and HCT116-R/DLD-1-R cells. (B) Cells were loaded with LysoTracker-LysoGreen for 30 min at 37°C. Cells were fixed and analyzed using a fluorescence microscope (×10 magnification). (C) Expression of PLD2 and Bcl-2 proteins was visualized by western blotting. (D) LAMP2A and PLD2 expression was measured by reverse transcription-quantitative PCR. The data were represented as the mean ± standard deviation from three independent experiments, *P<0.05. LAMP2A, lysosome-associated membrane protein 2A; CRC, colorectal cancer; R, resistance; PLD2, phospholipase D2.