Figure 6.

BBR regulates osteogenic and adipogenic differentiation via AMPK activation. The cells were incubated with BBR (10 µM), C.C (1 µM) or both, in the presence or absence of LPS (1 µg/ml), in osteogenic media for 7 days or in adipogenic media for 12days. Then, the cells were collected for ALP staining, ALP content assay or oil red O staining. The images of the ALP stained cells were obtained using a (A) camera and (B) microscope at ×200 magnification. (C) Relative ALP content levels of osteogenic cells were measured and normalized to control levels. The images of the oil red O stained cells were obtained using a (D) camera and (E) microscope at ×200 magnification. The data are presented as the mean ± standard deviation of three independent experiments. ***P<0.001, ****P<0.0001. C.C, compound C; BBR, berberine hydrochloride; LPS, lipopolysaccharide; ALP, alkaline phosphatase.