Table I.
Primer sequences for reverse transcription-quantitative PCR.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
|---|---|---|
| ALP | AACGTGGCCAAGAACATCATCA | TGTCCATCTCCAGCCGTGTC |
| RUNX2 | GCACCCAGCCCATAATAGA | TTGGAGCAAGGAGAACCC |
| OCN | TGAGGACCCTCTCTCTGCTC | GGGCTCCAAGTCCATTGTT |
| SPP1 | ATCTGAGTCCTTCACTG | GGGATACTGTTCATCAGAAA |
| FABP4 | GCGTAGAAGGGGACTTGGTC | TTCCTGTCATCTGGGGTGATT |
| ADIPSIN | CACGTGTGCGGTGGCACCCTG | CCCCTGCAAGTGTCCCTGCGGT |
| PPARγ | CCTTTACCACGGTTGATTTCTC | GGCTCTACTTTGATCGCACTTT |
| MCP-1 | TGCTGTCTCAGCCAGATGCAGTTA | AGAAGTGCTTGAGGTGGTTGTGGA |
| TNF-α | CCCAATCTGTGTCCTTCTAACT | CAGCGTCTCGTGTGTTTCT |
| Il-6 | GGTTTGCCGAGTAGACCTCA | GTGGCTAAGGACCAAGACCA |
| Il-1β | AAAGAAGGTGCTTGGGTCCT | CAGGAAGGCAGTGTCACTCA |
| GAPDH | CAGGGCTGCCTTCTCTTGT | TCCCGTTGATGACCAGCTTC |
ALP, alkaline phosphatase; Runx2, RUNX family transcription factor 2; Ocn, osteocalcin; Spp1, secreted phosphoprotein 1; Fabp4, fatty acid binding protein 4; Pparγ, peroxisome proliferator-activated receptorγ; MCP-1, monocyte chemoattractant protein-1.