Figure 1.

Detailed information of grouping and treatment. (A) Detailed diagram of tumor therapy. (B, C, D) The grouping at different stages. The dosage and frequency of treatment: SA-GM-CSF-anchored vaccine: 1×10⁶ cells/100 µl for each dose, once every 4 days for 5 doses. Soluble SA-GM-CSF: 100 ng/100 µl for each dose, once every 4 days for 5 doses. Ethanol-fixed MB49 cells: 1×10⁶ cells/100 µl for each dose, once every 4 days for 5 doses. PBS: 100 µl for each dose, once every 4 days for 5 doses. Anti-IFNγ antibody: 100 µg for each dose, once every 3 days for 6 doses. Anti-PD-1 antibody (eBioscience): 100 µg for each dose, once every 3 days for 6 doses. IgG antibody (eBioscience): 100 µg for each dose, once every 3 days for 6 doses. Anti-Tim-3 antibody (Invitrogen): 100 µg for each dose, once every 3 days for 6 doses. The routes of administration for the SA-GM-CSF-anchored vaccine, soluble SA-GM-CSF, ethanol-fixed MB49 cells, and PBS: intracutaneous injection; for the anti-IFNγ antibody, anti-PD-1 antibody, IgG antibody, and anti-Tim-3 antibody: intraperitoneal injection. ^*: when analyzing the expression of PD-L1 in the TME, anti-IFNγ antibody treatment was added.