Figure 3.

Expression of PD-1 on CD8⁺ TILs and PD-L1 expression in the TME after Anchored-GM-CSF vaccine treatment. (A) After treatment, TILs were isolated from tumor tissues of each group and purified by CD8a (Ly-2) MicroBeads. The sorting rate of CD8⁺TILs was detected by flow cytometry. (B) The CD8⁺TILs were stained with antibodies against PD-1, and PD-1 expression was assessed by flow cytometry (**P<0.01). (C) Tumor tissues from each group were harvested and stained with an anti-mPD-L1 antibody. Then, a goat antirabbit secondary antibody was used to assess PD-L1-positive cells. Blinded quantitation was performed in 10 different fields at 40× magnification (**P<0.01). All the experiments were replicated 3 times.