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. 2021 Jan 30;12(7):1967–1977. doi: 10.7150/jca.52455

Figure 4.

Figure 4

VEGF is a direct target of miR‑16 in cells. (A) The miR-16 levels of SKM-1 cells following miR-16 mimics /inhibitor (16-up/down) transfection for 48 h were analysed by qPCR. (B) The expression of VEGF protein of SKM-1 cells following miR-16 mimics /inhibitor transfection for 48 h was analysed by Western blotting analysis with β-actin as internal control. (C) Luciferase reporter assays were performed by co-transfection of pre-miR-16 oligonucleotide with a luciferase reporter gene containing wild-type 3'UTR of VEGF or mutated 3'UTR of VEGF. Expression of pre-miR-16 reduced luciferase activity of the wild-type reporter. * P<0.05, **P<0.01.