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. 2021 Jan 30;12(7):1915–1925. doi: 10.7150/jca.49200

Figure 3.

Figure 3

Mechanism dissection of how AR can decrease the ACP5 expression in the HCC cells: via altering the miR-325 expression. A-B. qRT-PCR was used to check related miRNAs expression after oeAR/shAR in HCC cells. C. Western blot assay was used to check oeAR/shAR efficiency in HCC cells. D-E. Wound-healing assay was used to check the migration capacity after adding miR-325 inhibitor/oemiR-325 in HCC cells. F-G. Transwell invasion assay was used to check invasion capacity after adding miR-325 inhibitor/oemiR-325 in HCC cells. H. Western blot assay was used to check ACP5 expression after adding miR-325 inhibitor in HA22T cells. I. Western blot assay was used to check ACP5 expression level after oemiR-325 in SK-HEP-1 cells. J. Wound-healing assay was used to check the migration capacity after shAR/oemiR-325 in HA22T cells. K. Western blot assay was used to check AR and ACP5 expression after shAR/oemiR-325 in HA22T cells. L. Transwell invasion assay was used to check invasion capacity after shAR/oemiR-325 in HA22T cells. M. Transwell invasion assay was used to check invasion capacity after oeAR/ adding miR-325 inhibitor in SK-HEP-1 cells. N. Wound-healing assay was used to check invasion capacity after oeAR/ adding miR-325 inhibitor in SK-HEP-1 cells. O. Western blot assay was used to check AR and ACP5 expression after oeAR/adding miR-325 inhibitor in SK-HEP-1 cells. All quantifications are mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant difference.