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. 2021 Mar 1;12(8):2430–2439. doi: 10.7150/jca.53385

Figure 3.

Figure 3

circNT5E sponges miR-502-5p in Bca cells. (A) Nucleo-cytoplasmic separation assay showed that circNT5E is mostly distributed in the cytoplasm. (B) circNT5E expression in T24 and SW780 cells transfected with pcNDA3.1-circNT5E. (C) qRT-PCR assay presented that circNT5E expression was augmented in Bca cells incubated with a specific circNT5E probe. (D, E) Only miR-502-5p was notably pulled down by a specific circNT5E probe in T24 and SW780 cells. (F, G) The luciferase activities of T24 and SW780 cells transfected with miR-502-5p mimics and luciferase reporter vectors. (H) miR-502-5p expression in T24 and SW780 cells transfected with sicircNT5E. (I) miR-502-5p expression in T24 and SW780 cells transfected with pcDNA3.1-circNT5E. *P <0.05, **P<0.01 and NS: none significant.