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. 2021 Feb 22;12(8):2206–2215. doi: 10.7150/jca.51139

Figure 4.

Figure 4

RO3306 inhibited HEC-1-B cell growth through induction of apoptosis and G2/M phase arrest. (A) HEC-1-B cells were treated with RO3306 at different concentrations of 0, 5 and 10 µM for 24 or 48 h. Apoptosis assay was performed with Annexin V-FITC/PI double staining using FACS analysis. (B) HEC-1-B cells were treated with RO3306 at different concentrations of 0, 5 and 10 µM for 24 or 48 h. Cell cycle assay was performed with PI staining using FACS analysis. The representative images of 3 independent experiments were shown. (C) Quantification of B. Results are the means (n = 3).