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. 2021 Mar 5;13(5):1127. doi: 10.3390/cancers13051127

Figure 6.

Figure 6

CK2 and IKAROS regulate sensitivity towards daunorubicin. CK2 overexpressing U937 (A,B) and THP-1 (C,D) were treated with 10 nM or 50 nM of daunorubicin for 48 hand stained with 7-AAD and Annexin V for flow cytometry to determine apoptosis. Flow plots (A,C) and percent apoptotic cells (early + late apoptosis) are shown in (B,D). Flow plots showing representative results from three replicates. The percentage of cells in the right upper and lower quadrant of each flow chart represents the percentage of late and early apoptotic cells, respectively. Q1-dead, Q2-late apoptosis, Q3-early apoptosis, Q4-live. (E) Cytotoxicity and drug response measured by MTT assay after treating CK2 overexpressing U937 and THP-1 cells and respective controls with various concentrations of daunorubicin for 48 h. p > 0.05 (ns); p < 0.05 (*); p < 0.001 (***); p < 0.0001 (****).