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. Author manuscript; available in PMC: 2021 Mar 19.
Published in final edited form as: Science. 2020 May 28;369(6499):54–59. doi: 10.1126/science.abb6151

Figure 4. AcrVIA1 enables full phage escape from type VIA CRISPR-Cas immunity.

Figure 4.

(A) Efficiency of plaquing (relative to the number of plaques formed in lawns of L. seeligeri ΔRM Δspc) of phages ϕLS46 or ϕLS46 ΔacrVIA1 in lawns of bacteria expressing spcA1, spcE1, spcE2 or all three (3 spc). Error bars represent SEM from 3 biological replicates. (B-F) Growth of L. seeligeri ΔRM Δspc (B), ΔRM ΩspcE1 (C), ΔRM ΩspcE2 (D), ΔRM ΩspcA1 (E) and ΔRM Ω3spc (F), measured as OD600 over time, infected with ϕLS46 or ϕLS46 ΔacrVIA1 phages, or uninfected. The average curves of three different replicates are reported, with +/− SEM values shown.