Figure 3.

Characterization of CycE-Luc2 reporter for the G1 phase in MCF-7 breast cancer cells. CycE-Luc2 plasmid was transiently transfected in MCF-7 cells. Then, CycE-luc2 expressing MCF-7 cells were synchronized by either 0.4 µg/mL nocodazole (A-C) or 2 mmol/L thymidine treatment (D-F). Later, the luciferase activity was measured at different time points (A, D). The change of cell content (B, E) and cell cycle distribution (C, F) were analyzed by flow cytometer. All the groups have four replicates and the experiments were repeated for three times. Error bars indicate standard error.