Skip to main content
. 2021 Jan 31;17(3):728–741. doi: 10.7150/ijbs.52101

Figure 4.

Figure 4

The degradation of CycE-Luc or CycE-Luc2 was mediated by proteasome proteolysis. (A and B) HeLa-CycE-Luc cells were transiently transfected with either Fbw7 siRNA or mock NC siRNA, cells lysed were used for analyzing CycE-Luc protein expression (A) and luciferase activity (B). (C and D) After HeLa-CycE-Luc cells were treated with the proteasome inhibitors MG132 (20 µm and 40 µm) for 6 hours. Cells lysates were used for analyzing the expression of CycE-Luc fusion protein by immunoblotting (C) and luciferase activity (D). (E and F) After HeLa-CycE-Luc2 clone 2 cells were treated with the proteasome inhibitors MG132 (20 µm) for 5 or 10 hours. Cells lysates were used for analyzing the expression of CycE-Luc2 fusion protein by immunoblotting (C) and luciferase activity (D). All the groups have four replicates and the experiments were repeated for three times. Error bars indicate standard error. *: p<0.05; ***: p<0.001.